In the context of blue-white screening,non-re | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(B) The blue-white screening method is based on the insertional inactivation of the $lacZ$ gene. $1$. The $lacZ$ gene encodes the enzyme $\beta$-galac

Vedclass · Accepted Answer

Non-recombinant bacteria contain beta-galactosidase.

Vedclass · Answer

(B) The blue-white screening method is based on the insertional inactivation of the $lacZ$ gene. $1$. The $lacZ$ gene encodes the enzyme $\beta$-galactosidase. $2$. In non-recombinant bacteria,the $lacZ$ gene is intact and functional,so the bacteria produce $\beta$-galactosidase. This enzyme reacts with the chromogenic substrate $X-gal$ to produce a blue color. $3$. In recombinant bacteria,the foreign $DNA$ is inserted into the $lacZ$ gene,causing insertional inactivation. Consequently,no functional $\beta$-galactosidase is produced,and the colonies remain white.

In the context of blue-white screening,non-recombinant bacterial colonies appear blue in contrast to white recombinant colonies because:

Similar Questions

You have identified a useful gene in bacteria. Make a flow chart of the steps that you would follow to transfer this gene to a plant.

$PCR$ (Polymerase Chain Reaction) technique is useful in:

$PCR$ (Polymerase Chain Reaction) is used for the diagnosis of which of the following diseases?

The transfer of protein from electrophoretic gel to nitrocellulose membrane is known as

Following enzymes/chemical/technique are used in the process of gel electrophoresis:
$I.$ sample $DNA$ is cut into fragments
$II.$ restriction endonucleases
$III.$ agarose gel
$IV.$ ethidium bromide
$V.$ $UV$-radiation
$VI.$ elution
Mark the correct sequence of their use.

Vedclass Test Series

Exam Paper Generator

Online Exam Module