In which medium did Meselson and Stahl first | vedclass

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(B) Meselson and Stahl performed an experiment to prove that $DNA$ replication is semi-conservative. They first grew $E. coli$ in a medium containing

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$^{15}NH_4Cl$

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(B) Meselson and Stahl performed an experiment to prove that $DNA$ replication is semi-conservative. They first grew $E. coli$ in a medium containing $^{15}NH_4Cl$ as the only nitrogen source for many generations. This resulted in the incorporation of the heavy isotope $^{15}N$ into the newly synthesized $DNA$ of the bacteria. After this,they transferred the cells into a medium with normal $^{14}NH_4Cl$ and took samples at various time intervals to observe the density of the $DNA$ using $CsCl$ density gradient centrifugation.

In which medium did Meselson and Stahl first culture $E. coli$?

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