The $Western$ $Blot$ technique is used for which of the following?

How many types of primers are required for the amplification of a gene in $PCR$?

The optimum temperature for polymerisation in $PCR$ is $....$ while the enzyme responsible for the mentioned step can tolerate temperatures up to $....$. Select the correct option according to the blanks.

Explain how the synthesis of a gene of interest is performed using $PCR$.

In which step of $PCR$ do the primers anneal to the $DNA$ template?

When foreign $\text{DNA}$ is inserted into any vector,it results in inactivation of any marker gene. This is used for selection of $......\ :-$