The full form of $PCR$ is ...........

Which impurities are released along with $DNA$ when cells are broken down for $DNA$ isolation?

Arrange the steps of $\text{rDNA}$ technology in the correct order$:-$
$(I.)$ Extraction of the desired gene product.
$(II.)$ Amplification of the gene of interest.
$(III.)$ Isolation of the desired $\text{DNA}$ fragment.
$(IV.)$ Ligation of the $\text{DNA}$ fragment into a vector.
$(V.)$ Insertion of $\text{rDNA}$ into the host.
The correct order is$:-$

Biolistics (gene gun) is suitable for:

At which stage of $PCR$,does the template join with the primer?

What is used for the large-scale production of products in biotechnology?