During PCR,denaturation of DNA is achieved by | vedclass

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(A) The $PCR$ (Polymerase Chain Reaction) process consists of three main steps: denaturation,annealing,and extension.$1$. Denaturation: The double-str

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High temperature

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(A) The $PCR$ (Polymerase Chain Reaction) process consists of three main steps: denaturation,annealing,and extension.$1$. Denaturation: The double-stranded $DNA$ template is heated to a high temperature (approximately $94-98^{\circ}C$). This high temperature breaks the hydrogen bonds between the complementary base pairs,resulting in the separation of the two strands of $DNA$.$2$. Annealing: The temperature is lowered to allow primers to bind to the single-stranded $DNA$.$3$. Extension: The temperature is adjusted for the $DNA$ polymerase to synthesize the new $DNA$ strand.Therefore,denaturation is achieved by high temperature.

List $I$	List $II$
a) Visualization of $DNA$ in gel electrophoresis	i) Chitinase
b) Precipitation of $DNA$	ii) Lysozyme
c) Breaking of cell wall of bacteria	iii) Chilled ethanol
d) Breaking of cell wall of fungus	iv) Ethidium bromide

During $PCR$,denaturation of $DNA$ is achieved by which of the following?

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Match List $I$ with List $II$:
List $I$ List $II$
a) Visualization of $DNA$ in gel electrophoresis i) Chitinase
b) Precipitation of $DNA$ ii) Lysozyme
c) Breaking of cell wall of bacteria iii) Chilled ethanol
d) Breaking of cell wall of fungus iv) Ethidium bromide

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