If foreign DNA is inserted into pBR322 at the | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(B) The plasmid $pBR322$ contains two antibiotic resistance genes: $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance).The $Pst \, I

Vedclass · Accepted Answer

It will grow in tetracycline-containing medium but not in ampicillin-containing medium.

Vedclass · Answer

(B) The plasmid $pBR322$ contains two antibiotic resistance genes: $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance).The $Pst \, I$ restriction site is located within the $amp^R$ gene.When foreign $DNA$ is inserted at the $Pst \, I$ site,the $amp^R$ gene undergoes insertional inactivation,meaning it becomes non-functional.As a result,the bacteria containing this recombinant plasmid will lose their resistance to ampicillin but will retain their resistance to tetracycline.Therefore,the bacteria will grow in a tetracycline-containing medium but will fail to grow in an ampicillin-containing medium.

If foreign $DNA$ is inserted into $pBR322$ at the $Pst \, I$ site and this plasmid is introduced into bacteria,then:

Similar Questions

Restriction enzymes are:

The given figure is the diagrammatic representation of the $E. coli$ vector $pBR322$. Which one of the given options correctly identifies its certain component$(s)$?

$A$ piece of foreign $DNA$ can replicate if it is linked to which of the following?

$pBR322$ contains the recognition sites for which of the following endonucleases at the $amp^R$ site?

The introduction of $T-DNA$ into plants involves

Vedclass Test Series

Exam Paper Generator

Online Exam Module