In pBR322,foreign DNA is inserted using the r | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(A) The plasmid $pBR322$ contains two antibiotic resistance genes: $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance).The restricti

Vedclass · Accepted Answer

The bacteria will grow on ampicillin-containing medium but not on tetracycline-containing medium.

Vedclass · Answer

(A) The plasmid $pBR322$ contains two antibiotic resistance genes: $amp^R$ (ampicillin resistance) and $tet^R$ (tetracycline resistance).The restriction site for the enzyme $Cla\, I$ is located within the $tet^R$ gene.When foreign $DNA$ is inserted at the $Cla\, I$ site,the $tet^R$ gene undergoes insertional inactivation,meaning it becomes non-functional.Consequently,the bacteria containing this recombinant plasmid will lose their resistance to tetracycline.However,the $amp^R$ gene remains intact and functional.Therefore,the transformed bacteria will be able to grow on ampicillin-containing medium but will fail to grow on tetracycline-containing medium.

In $pBR322$,foreign $DNA$ is inserted using the restriction enzyme $Cla\, I$. If this recombinant plasmid is introduced into bacteria,which of the following will occur regarding growth on antibiotic-containing media?

Similar Questions

$A$ number of bacteria with recombinant $DNA$ of the same type form:

If a recombinant $DNA$ molecule carrying an ampicillin resistance gene is introduced into an $E. coli$ cell and the host cells are spread on an agar plate containing ampicillin,then...

To introduce recombinant $DNA$ into a host cell,it is first kept on ice,then placed at ........ temperature,and finally placed back on ice. (in $^{\circ} C$)

In Southern blotting,which of the following is separated by gel electrophoresis?

Vedclass Test Series

Exam Paper Generator

Online Exam Module