What is the first step in the Southern Blotti | vedclass

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(D) The Southern Blotting technique is a method used for detection of specific $DNA$ sequences in $DNA$ samples. The steps involved are: $1$. Isolatio

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Isolation of $DNA$ from nucleated cells, such as obtaining $DNA$ from a crime scene

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(D) The Southern Blotting technique is a method used for detection of specific $DNA$ sequences in $DNA$ samples. The steps involved are: $1$. Isolation of $DNA$ from the sample (e.g., blood, hair, or crime scene evidence). $2$. Digestion of $DNA$ using restriction endonucleases. $3$. Separation of $DNA$ fragments by gel electrophoresis. $4$. Transfer (blotting) of separated $DNA$ fragments to a synthetic membrane (nitrocellulose or nylon). $5$. Hybridization using a labeled probe. $6$. Detection of hybridized fragments using autoradiography. Therefore, the first step is the isolation of $DNA$ from the source.

What is the first step in the Southern Blotting technique?

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