Which of the following is used for direct gen | vedclass

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(D) Direct gene transfer,also known as physical gene transfer,involves introducing foreign $DNA$ directly into the host cell without the use of a vect

Vedclass · Accepted Answer

All of the above

Vedclass · Answer

(D) Direct gene transfer,also known as physical gene transfer,involves introducing foreign $DNA$ directly into the host cell without the use of a vector. $1$. Microinjection: $DNA$ is directly injected into the nucleus of an animal cell using a fine glass needle. $2$. Electroporation: High-voltage electrical pulses are used to create temporary pores in the cell membrane,allowing $DNA$ to enter. $3$. Biolistics (Gene gun): Microscopic particles of gold or tungsten coated with $DNA$ are bombarded into target cells. Since all three methods are techniques for direct gene transfer,the correct answer is $D$.

Which of the following is used for direct gene transfer?

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