Insertional inactivation is performed by which enzyme?

Which of the following organelles is related to genetic engineering?

Given below are two statements:
Statement $I:$
Restriction endonucleases recognise specific sequences to cut $DNA$,known as palindromic nucleotide sequences.
Statement $II:$
Restriction endonucleases cut the $DNA$ strand a little away from the centre of the palindromic site.
In the light of the above statements,choose the most appropriate answer from the options given below:

Which metal is used in particle bombardment (biolistics) for gene transfer?

Match the enzymes in Column-$I$ with their respective functions in Column-$II$.

Column-$I$	Column-$II$
$P$. Restriction endonuclease	$I$. Synthesizes $DNA$ copies
$Q$. $DNA$ polymerase	$II$. Cleaves $DNA$ molecules
$R$. $DNA$ ligase	$III$. Joins $DNA$ molecules

In the $pBR322$ plasmid,the ampicillin and tetracycline resistance genes contain recognition sites for how many restriction enzymes,respectively?