In which year did Cohen and Boyer isolate the antibiotic resistance gene by cutting out a piece of $DNA$ from a plasmid?

Mention the features that are required to facilitate cloning into a vector.

Which technology involves altering the chemistry of genetic material ($DNA$ and $RNA$) to introduce it into host organisms and thus change the phenotype of the host organism?

An alien $DNA$ is linked with the origin of replication, so that this alien piece of $DNA$ can replicate and multiply itself in the host organism. This can also be called ......

The critical research areas of biotechnology are:
$I$. Providing the best catalyst as an improved organism,usually a microbe or pure enzyme.
$II$. Creating optimal conditions through engineering for a catalyst to act.
$III$. Downstream processing technologies.
$IV$. Multiple Ovulation Embryo Transfer Technology $(MOET)$.
Which of the statements given above are correct?

Give general information about Biotechnology.