The Feulgen reaction in DNA is observed as a | vedclass

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(A) The Feulgen reaction is a staining technique used to identify chromosomal material or $DNA$ in cell nuclei. It involves mild acid hydrolysis of $D

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Aldehydes produced by acid hydrolysis

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(A) The Feulgen reaction is a staining technique used to identify chromosomal material or $DNA$ in cell nuclei. It involves mild acid hydrolysis of $DNA$ using $1M$ $HCl$ at $60^{\circ}C$. This process removes purine bases from the $DNA$ molecule,which exposes the aldehyde groups of the deoxyribose sugar. These free aldehyde groups then react with the Schiff's reagent to produce a magenta or purple color,confirming the presence of $DNA$.

The Feulgen reaction in $DNA$ is observed as a result of:

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