What is done during the process of hardening?

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(A) Hardening is a critical step in micropropagation where tissue-cultured plantlets are gradually acclimatized to the external environment. Initially

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Plantlets are kept in a place with low light and high humidity for a short period.

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(A) Hardening is a critical step in micropropagation where tissue-cultured plantlets are gradually acclimatized to the external environment. Initially,these plantlets are grown in a controlled,high-humidity,and low-light environment within the laboratory. During hardening,they are slowly exposed to lower humidity and higher light intensity to prepare them for survival in the field or greenhouse conditions. This process prevents transplant shock and ensures the survival of the plantlets.

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