Which of the following events is essential in the plant tissue culture technique?

The first successful pollen culture was performed by:

Which of the following is used in tissue culture?

Which of the following substances is $NOT$ used for the formation and maintenance of callus in plant tissue culture?

Arrange the following steps of somatic hybridisation in a correct sequence.
$A$. Digestion of cell walls.
$B$. Isolation of naked protoplasts.
$C$. Fusion of protoplasts to get hybrid protoplast.
$D$. Isolation of single cells from two different varieties of plants.
$E$. Growing of hybrid protoplast to form a new plant.
Choose the correct answer from the options given below:

Tissue culture technique can produce an infinite number of new plants from a small parental tissue. The economic importance of the technique is in raising