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(D) Tissue culture is a technique of growing plant cells,tissues,or organs in a sterile,controlled environment. $1$. Maintaining a sterile environment

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All of the above

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(D) Tissue culture is a technique of growing plant cells,tissues,or organs in a sterile,controlled environment. $1$. Maintaining a sterile environment is crucial to prevent contamination by microorganisms like bacteria and fungi. $2$. Providing an optimal temperature (often around $24^oC$ to $28^oC$) is necessary for proper metabolic activity and growth. $3$. $A$ suitable culture medium containing essential nutrients,vitamins,and plant growth regulators is required to support the growth of the explant. Therefore,all these factors are essential for successful tissue culture.

What are the essential requirements to be considered for tissue culture?

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