PGA as the first CO_2 fixation product was di | vedclass

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(D) The correct answer is $D$. Calvin,Benson,and their colleagues in California,$U.S.A.$,conducted experiments using radioactive $^{14}C$ in carbon di

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alga

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(D) The correct answer is $D$. Calvin,Benson,and their colleagues in California,$U.S.A.$,conducted experiments using radioactive $^{14}C$ in carbon dioxide to feed $Chlorella$ and $Scenedesmus$ (which are algae).Radioactive carbon,$^{14}C$,has a half-life of $5568$ years,allowing the path of $CO_2$ fixation to be traced.Algal suspension,while performing photosynthesis,was supplied with $^{14}CO_2$.The algae were killed at specific intervals in near-boiling methanol,which immediately stopped photosynthetic activity due to the denaturation of enzymes.After evaporating the alcohol and crushing the algae,the resulting paste was analyzed using two-dimensional paper chromatography.Calvin and his co-workers discovered that after three seconds,radioactivity appeared in phosphoglyceric acid $(PGA)$.Thus,$PGA$ was identified as the first stable product of photosynthesis in algae.

$PGA$ as the first $CO_2$ fixation product was discovered in photosynthesis of

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