Assertion : Restriction enzymes cut the stran | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(C) Restriction enzymes,a type of endonuclease,function by inspecting the length of a $DNA$ sequence. Once they find a specific recognition sequence,t

Vedclass · Accepted Answer

If the Assertion is correct but Reason is incorrect.

Vedclass · Answer

(C) Restriction enzymes,a type of endonuclease,function by inspecting the length of a $DNA$ sequence. Once they find a specific recognition sequence,they bind to it and cut each of the two strands of the double helix at specific points,leaving single-stranded portions at the ends. This results in overhanging stretches called sticky ends. These are named so because they form hydrogen bonds with their complementary counterparts; i.e.,they can join similar complementary ends of $DNA$ fragments from other sources with the help of the enzyme $DNA$ ligase. Therefore,the stickiness of the ends facilitates the action of the enzyme $DNA$ ligase,not $DNA$ polymerase. Thus,the Assertion is correct,but the Reason is incorrect.

Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.
Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.

Similar Questions

Which organism is used as a vector to introduce specific genes into host plants?

What was linked with the plasmid in the construction of the first $r-DNA$?

Which of the following key factors makes a plasmid a suitable vector in genetic engineering?

The introduction of $T-DNA$ into plants involves

$DNA$ can be studied after staining with . . . . . . in various fields.

Vedclass Test Series

Exam Paper Generator

Online Exam Module

Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.