Differentiation of shoot is controlled by

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(B) The differentiation of plant tissues in tissue culture is regulated by the balance between auxin and cytokinin. High concentrations of cytokinin r

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High cytokinin : auxin ratio

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(B) The differentiation of plant tissues in tissue culture is regulated by the balance between auxin and cytokinin. High concentrations of cytokinin relative to auxin promote shoot development (caulogenesis). Conversely,high concentrations of auxin relative to cytokinin promote root development (rhizogenesis). Therefore,a high cytokinin to auxin ratio is responsible for shoot differentiation.

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