During the process of gene amplification usin | vedclass

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(C) The $PCR$ (Polymerase Chain Reaction) process consists of three main steps: $1$. Denaturation,$2$. Annealing,and $3$. Extension.In the first step,

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Denaturation

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(C) The $PCR$ (Polymerase Chain Reaction) process consists of three main steps: $1$. Denaturation,$2$. Annealing,and $3$. Extension.In the first step,Denaturation,the double-stranded $DNA$ template is heated to a very high temperature (approximately $94-98^{\circ}C$) to separate the two strands by breaking the hydrogen bonds between the complementary base pairs.If this high temperature is not maintained at the beginning,the $DNA$ strands will not separate,and the subsequent steps (Annealing and Extension) cannot occur.Therefore,Denaturation is the first step that will be affected.

During the process of gene amplification using $PCR$,if very high temperature is not maintained in the beginning,then which of the following steps of $PCR$ will be affected first?

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