From the given combination of steps in PCR,id | vedclass

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Question

(A) The Polymerase Chain Reaction $(PCR)$ consists of three main steps: Denaturation,Annealing,and Extension.$1$. Denaturation: The double-stranded $D

Vedclass · Accepted Answer

Extension only

Vedclass · Answer

(A) The Polymerase Chain Reaction $(PCR)$ consists of three main steps: Denaturation,Annealing,and Extension.$1$. Denaturation: The double-stranded $DNA$ is heated to high temperatures $(94-98^{\circ}C)$ to separate the strands. This is a physical process,not enzyme-dependent.$2$. Annealing: Primers bind to the single-stranded $DNA$ template at lower temperatures $(50-65^{\circ}C)$. This is also not enzyme-dependent.$3$. Extension: The enzyme $Taq$ $DNA$ polymerase adds nucleotides to the primer,extending the new $DNA$ strand. This step is strictly dependent on the activity of the $DNA$ polymerase enzyme.Therefore,the correct answer is Extension only.

Column-$I$	Column-$II$
$(a)$ Lysozyme	$(i)$ Fat
$(b)$ Cellulase	$(ii)$ Protein
$(c)$ Chitinase	$(iii)$ Bacteria
$(d)$ Lipase	$(iv)$ Fungi (Penicillium)
	$(v)$ Plant (Mangifera indica)

From the given combination of steps in $PCR$,identify the enzyme-dependent step.

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Match the columns:

Column-$I$ Column-$II$

$(a)$ Lysozyme $(i)$ Fat

$(b)$ Cellulase $(ii)$ Protein

$(c)$ Chitinase $(iii)$ Bacteria

$(d)$ Lipase $(iv)$ Fungi (Penicillium)

$(v)$ Plant (Mangifera indica)

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