Gene therapy is an attempt to correct a genetic defect by providing a normal gene into the individual. By this,the normal function can be restored. An alternate method would be to provide the gene product (protein/enzyme) known as enzyme replacement therapy,which would also restore the function. Which in your opinion is a better option? Give reason for your answer.

Given below is the sequence of events in the production of human insulin by Eli Lilly.
$(a)$ Preparation of two $DNA$ sequences corresponding to chain $A$ and $B$ of human insulin.
$(b)$ Production of chain $A$ and $B$ separately.
$(c)$ Introduction of chains in plasmids of $E. coli$.
$(d)$ Extraction of chain $A$ and $B$ and combining them by creating disulphide bonds.
Choose the correct sequence of events.

Statements related to human insulin are given below. Which statement$(s)$ is/are correct about genetically engineered insulin?
$(a)$ Pro-hormone insulin contains an extra stretch of $C$-peptide.
$(b)$ $A$-peptide and $B$-peptide chains of insulin were produced separately in $E. coli$,extracted,and combined by creating disulfide bonds between them.
$(c)$ Insulin used for treating diabetes was extracted from cattle and pigs.
$(d)$ Pro-hormone insulin needs to be processed for converting into a mature and functional hormone.
$(e)$ Some patients develop allergic reactions to the foreign insulin.
Choose the most appropriate answer from the options given below:

The International Centre for Genetic Engineering and Biotechnology $(ICGEB)$ established by the United Nations Organization $(UNO)$ is located at

Who was the first patient who was given gene therapy? Why was the given treatment recurrent in nature?

Which among the following is used to treat Emphysema?