Given below are four statements pertaining to the separation of $DNA$ fragments using gel electrophoresis. Identify the incorrect statements:
$(a)$ $DNA$ is a negatively charged molecule and so it is loaded on the gel towards the Anode terminal.
$(b)$ $DNA$ fragments travel along the surface of the gel,whose concentration does not affect the movement of $DNA$.
$(c)$ The smaller the size of the $DNA$ fragment,the larger is the distance it travels through it.
$(d)$ Pure $DNA$ can be visualized directly by exposing it to $UV$ radiation.
Choose the correct answer from the options given below:
$(a)$ $DNA$ is a negatively charged molecule and so it is loaded on the gel towards the Anode terminal.
$(b)$ $DNA$ fragments travel along the surface of the gel,whose concentration does not affect the movement of $DNA$.
$(c)$ The smaller the size of the $DNA$ fragment,the larger is the distance it travels through it.
$(d)$ Pure $DNA$ can be visualized directly by exposing it to $UV$ radiation.
Choose the correct answer from the options given below:
- A$(a), (c)$ and $(d)$
- B$(a), (b)$ and $(c)$
- C$(b), (c)$ and $(d)$
- D$(a), (b)$ and $(d)$
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Select the correct sequence for $PCR$ (Polymerase Chain Reaction).
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Select the option that represents the correct sequence of steps in $r-DNA$ technology.
$(1)$ Insertion of $r-DNA$ into the host
$(2)$ Isolation of $DNA$
$(3)$ Ligation of $DNA$ fragment into the vector
$(4)$ Culturing the host cells in a medium at a large scale and extraction of the desired product
$(5)$ Isolation of the desired $DNA$ fragment
$(6)$ Fragmentation of $DNA$ by restriction endonucleases
$(1)$ Insertion of $r-DNA$ into the host
$(2)$ Isolation of $DNA$
$(3)$ Ligation of $DNA$ fragment into the vector
$(4)$ Culturing the host cells in a medium at a large scale and extraction of the desired product
$(5)$ Isolation of the desired $DNA$ fragment
$(6)$ Fragmentation of $DNA$ by restriction endonucleases
Medium
View SolutionRefer to the following statements for agarose-gel electrophoresis:
$(a)$ The $\text{DNA}$ fragments resolve according to their size.
$(b)$ The separated $\text{DNA}$ fragment can be visualised only after staining with ethidium bromide followed by exposure to $UV$ radiation.
$(c)$ $\text{DNA}$ is always loaded into the well.
$(d)$ The smallest fragment of $\text{DNA}$ is near the well.
Choose the correct statements:
$(a)$ The $\text{DNA}$ fragments resolve according to their size.
$(b)$ The separated $\text{DNA}$ fragment can be visualised only after staining with ethidium bromide followed by exposure to $UV$ radiation.
$(c)$ $\text{DNA}$ is always loaded into the well.
$(d)$ The smallest fragment of $\text{DNA}$ is near the well.
Choose the correct statements:
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