Having become an expert on gel electrophoresi | vedclass

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(A) $DNA$ molecules are negatively charged due to the phosphate backbone. In gel electrophoresis,$DNA$ fragments are loaded into wells near the negati

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Near the positive electrode,farthest away from the wells

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(A) $DNA$ molecules are negatively charged due to the phosphate backbone. In gel electrophoresis,$DNA$ fragments are loaded into wells near the negative electrode (cathode). When an electric field is applied,the $DNA$ fragments migrate towards the positive electrode (anode). The gel matrix acts as a molecular sieve,where smaller fragments move faster and travel further through the pores of the gel compared to larger fragments. Therefore,the smallest $DNA$ segments are found farthest from the wells,near the positive electrode.

Having become an expert on gel electrophoresis,you are asked to examine a gel for a colleague. Where would you find the smallest segment of $DNA$?

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