How can a recombinant protein be obtained?

The different basic steps of genetic engineering are given below randomly:
$I.$ Identification of $DNA$ with desirable genes
$II.$ Gene transfer
$III.$ Maintenance of $DNA$ in host and gene cloning
$IV.$ Introduction of $DNA$ into host to form recombinant $DNA$
Which of the following represents the correct sequence of steps?

The optimum temperature for polymerisation in $PCR$ is $....$ while the enzyme responsible for the mentioned step can tolerate temperatures up to $....$. Select the correct option according to the blanks.

Arrange the steps of $\text{rDNA}$ technology in the correct order$:-$
$(I.)$ Extraction of the desired gene product.
$(II.)$ Amplification of the gene of interest.
$(III.)$ Isolation of the desired $\text{DNA}$ fragment.
$(IV.)$ Ligation of the $\text{DNA}$ fragment into a vector.
$(V.)$ Insertion of $\text{rDNA}$ into the host.
The correct order is$:-$

In the above represented plasmid,an alien piece of $\text{DNA}$ is inserted at the $\text{EcoRI}$ site. Which of the following strategies will be chosen to select the recombinant colonies?

How many basic steps are involved in the creation of a $GMO$?