Identify and explain steps $A$,$B$,and $C$ in the $PCR$ diagram given below.

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(N/A) $PCR$ stands for Polymerase Chain Reaction. It is a technique used to amplify a specific segment of $DNA$ in vitro. Each cycle of $PCR$ consists of three main steps:
$A$: Denaturation: The double-stranded $DNA$ is heated to a high temperature (about $94-98^{\circ}C$),which causes the two strands to separate into single-stranded $DNA$.
$B$: Annealing: The temperature is lowered (typically $50-65^{\circ}C$) to allow the two oligonucleotide primers to bind (anneal) to their complementary sequences on the single-stranded $DNA$ templates.
$C$: Extension: The temperature is adjusted (usually $72^{\circ}C$) to allow the thermostable $DNA$ polymerase (e.g.,$Taq$ polymerase) to synthesize a new $DNA$ strand by adding nucleotides to the primers,using the original $DNA$ as a template. This process is repeated for many cycles to achieve exponential amplification of the target $DNA$ segment.

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Given below are two statements.
Statement $I$ - The isolated $DNA$ of donor organism is cleaved by suitable restriction endonuclease to form $DNA$ fragments with cohesive staggered or blunt ends.
Statement $II$ - The foreign $DNA$ or passenger $DNA$ can also be obtained directly from genomic or cDNA library.
In the light of above statements,select the correct option given below:

In a chemical process,the $DNA$ fragments produced by restriction endonucleases can be separated by:

How does one visualise $DNA$ on an agarose gel?

What is the criterion for the movement of $DNA$ fragments on an agarose gel during gel electrophoresis?

Assertion : The uptake of $DNA$ during transformation is an active,energy-requiring process.
Reason : Transformation occurs in only those bacteria,which possess the enzymatic machinery involved in the active uptake and recombination.

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