In the case of polymerase chain reaction (PCR | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(A) The polymerase chain reaction $(PCR)$ consists of three main steps:$1$. Denaturation: The double-stranded $DNA$ is heated to approximately $94^{\c

Vedclass · Accepted Answer

$A-94^{\circ}C, B-40^{\circ}C, C-72^{\circ}C$

Vedclass · Answer

(A) The polymerase chain reaction $(PCR)$ consists of three main steps:$1$. Denaturation: The double-stranded $DNA$ is heated to approximately $94^{\circ}C$ to separate the strands.$2$. Annealing: Primers are allowed to anneal to the single-stranded $DNA$ templates at a lower temperature,typically between $40^{\circ}C$ and $60^{\circ}C$.$3$. Extension: The $DNA$ polymerase enzyme synthesizes the new $DNA$ strand at an optimal temperature of $72^{\circ}C$.Therefore,the correct sequence of temperatures is $A-94^{\circ}C, B-40^{\circ}C, C-72^{\circ}C$.

In the case of polymerase chain reaction $(PCR)$,what are the temperatures required for the following steps?
$A.$ Denaturation
$B.$ Annealing
$C.$ Extension

Similar Questions

Which of the following methods are collectively referred to as downstream processing?

Why are stirred-tank bioreactors specifically designed?

Give scientific reasons: The products prepared by recombinant $DNA$ technology have to undergo downstream processing.

What happens if a foreign $DNA$ fragment is inserted into the coding sequence of the $\beta$-galactosidase enzyme in a plasmid containing the coding sequence for the $\beta$-galactosidase enzyme?

$A$ : Taq Polymerase is involved in $PCR$ technique.
$R$ : This enzyme remains active during the high temperature including denaturation of double stranded $DNA$.

Vedclass Test Series

Exam Paper Generator

Online Exam Module

In the case of polymerase chain reaction $(PCR)$,what are the temperatures required for the following steps?$A.$ Denaturation$B.$ Annealing$C.$ Extension