In the initial stages of protoplast culture,s | vedclass

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(D) Protoplasts are plant cells that have had their cell walls removed. Because they lack the rigid cell wall,they are highly susceptible to bursting

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As an osmotic stabilizer

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(D) Protoplasts are plant cells that have had their cell walls removed. Because they lack the rigid cell wall,they are highly susceptible to bursting due to osmotic pressure changes. Sorbitol or mannitol are non-metabolizable sugar alcohols added to the culture medium to maintain the osmotic balance,acting as osmotic stabilizers to prevent the protoplasts from lysing.

In the initial stages of protoplast culture,sorbitol or mannitol is added:

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