Plasmid of which bacterium was first time used in recombinant $DNA$ technology?

The introduction of $T-DNA$ into plants involves

Endonuclease . . . . . . .

The diagram below represents a schematic of recombinant $DNA$ technology. Which enzymes are responsible for the processes $P$ and $Q$ respectively?
$\quad\quad P\quad\quad\quad Q$

Which enzyme is used to digest the cell wall of fungi?

The enzyme known as restriction endonuclease: