Restriction enzymes may be used for:

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(D) Restriction enzymes,also known as molecular scissors,are essential tools in biotechnology.$1$. They are used to cut $DNA$ at specific recognition

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All the above

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(D) Restriction enzymes,also known as molecular scissors,are essential tools in biotechnology.$1$. They are used to cut $DNA$ at specific recognition sequences,which is the first step in creating recombinant $DNA$ molecules.$2$. They are used in gene mapping by creating specific $DNA$ fragments (Restriction Fragment Length Polymorphism - $RFLP$) that help in locating genes on chromosomes.$3$. They are used in the diagnosis of genetic diseases by identifying mutations that alter restriction sites,allowing for the detection of specific genetic disorders.Therefore,all the given options are correct applications of restriction enzymes.

Column-$I$	Column-$II$
$(A) E$	$(1) 1^{st}$ in order of identification
$(B) co$	$(2)$ Name of genus
$(C) R$	$(3)$ Name of species
$(D) I$	$(4)$ Name of strain

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