Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
$(i)$ Synthesis of gene $(DNA)$ for human insulin artificially.
(ii) Culturing recombinant $E. coli$ in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
$(v)$ Introduction of recombinant plasmid into $E. coli$.
(vi) Extraction of recombinant gene product from $E. coli$.

• A
  $(ii), (i), (iv), (iii), (v), (vi)$
• B
  $(i), (iii), (v), (vi), (ii), (iv)$
• C
  $(i), (iv), (v), (ii), (vi), (iii)$
• D
  $(iii), (v), (ii), (i), (vi), (iv)$