The diagram below refers to PCR. Identify the | vedclass

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(A) The $PCR$ (Polymerase Chain Reaction) process involves three major steps:$(i)$ Denaturation $(A)$: The double-stranded $DNA$ is heated to $94-96^{

Vedclass · Accepted Answer

$A-$Denaturation at $94-96^{\circ} C$,$B-$Annealing at $40-60^{\circ} C$,$C-$Extension through Taq polymerase at $72^{\circ} C$,$D-$Amplified

Vedclass · Answer

(A) The $PCR$ (Polymerase Chain Reaction) process involves three major steps:$(i)$ Denaturation $(A)$: The double-stranded $DNA$ is heated to $94-96^{\circ} C$ to separate it into single strands.$(ii)$ Annealing $(B)$: The temperature is lowered to $40-60^{\circ} C$ to allow primers to bind to the complementary sequences on the single-stranded $DNA$ templates.$(iii)$ Extension $(C)$: The temperature is raised to $72^{\circ} C$,allowing the thermostable Taq polymerase to synthesize new $DNA$ strands by adding nucleotides to the primers.After $30$ cycles $(D)$,the target $DNA$ region is significantly amplified.

The diagram below refers to $PCR$. Identify the steps $A, B$ and $C$ and select the correct option.

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