The membrane of which one of the following microorganisms is three-layered?

Read the following statements:
$A.$ Mango is included under order 'Poales'.
$B.$ Chemosynthetic autotrophic bacteria oxidize various inorganic substances such as nitrite and ammonia and use the released energy for their $ATP$ production.
$C.$ Members of Ascomycetes are parasitic only.
$D.$ In one citric acid cycle,$2 NADH_2$ and $1 FADH_2$ are formed.
$E.$ Pyruvic acid $+ CoA + NAD^+ \xrightarrow{\text{Pyruvate dehydrogenase}} \text{Acetyl } CoA + CO_2 + NADH + H^+$.
Choose the correct option:

$Thermococcus$,$Methanococcus$ and $Methanobacterium$ exemplify:

There is no alternation of generation in $Escherichia$ $coli$ because of the absence of

Which kingdom is characterized by the presence of a rigid cell wall and nucleoprotein as genetic material?

Mark the odd one $w.r.t.$ cell wall.