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(A) The Calvin cycle was elucidated by Melvin Calvin and his colleagues using the radioactive isotope technique. They used $^{14}C$ (a radioactive iso

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Radioactive isotope technique

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(A) The Calvin cycle was elucidated by Melvin Calvin and his colleagues using the radioactive isotope technique. They used $^{14}C$ (a radioactive isotope of carbon) in algal photosynthesis studies (specifically with Chlorella and Scenedesmus) to trace the path of carbon fixation,which led to the discovery of the $C_3$ cycle.

The methodology,which has been most useful for investigating the Calvin cycle,is

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