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(A) Tissue culture is a technique of growing plant cells,tissues,or organs in a sterile environment on a nutrient medium.The nutrient medium (such as

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$I, II, III, IV$ and $V$

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(A) Tissue culture is a technique of growing plant cells,tissues,or organs in a sterile environment on a nutrient medium.The nutrient medium (such as Murashige and Skoog medium) must provide all the essential components for the growth and development of the explant.These components include:$1$. Carbon source: Sucrose is the most commonly used carbon source.$2$. Inorganic salts: Essential for mineral nutrition (macro and micronutrients).$3$. Growth regulators: Auxins and cytokinins are added to control morphogenesis and cell division.$4$. Vitamins: Essential for metabolic processes.$5$. Amino acids: Often added as a nitrogen source to support rapid growth.Therefore,all the listed components ($I, II, III, IV$,and $V$) are required in the nutrient medium.

The nutrient medium for tissue culture should have:
$I$. Sucrose
$II$. Inorganic salts
$III$. Growth regulators
$IV$. Vitamins
$V$. Amino acids
Choose the correct option.

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The nutrient medium for tissue culture should have:$I$. Sucrose$II$. Inorganic salts$III$. Growth regulators$IV$. Vitamins$V$. Amino acidsChoose the correct option.