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(D) Tissue culture is a technique of growing plant cells,tissues,or organs in a sterile,controlled environment on a nutrient medium.The nutrient mediu

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$I, II, III$ and $IV$

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(D) Tissue culture is a technique of growing plant cells,tissues,or organs in a sterile,controlled environment on a nutrient medium.The nutrient medium (often called Murashige and Skoog medium or $MS$ medium) must provide all the essential components for the growth and development of the explant.$I.$ Sucrose acts as a carbon and energy source.$II.$ Inorganic salts provide essential macro and micronutrients (minerals) required for plant metabolism.$III.$ Amino acids serve as a nitrogen source for protein synthesis.$IV.$ Vitamins (such as thiamine,nicotinic acid,and pyridoxine) are essential co-factors for enzymatic reactions.Therefore,all these components are necessary for the successful growth of plant tissue in vitro.

The nutrient medium for tissue culture should have:
$I.$ Sucrose
$II.$ Inorganic Salts
$III.$ Amino acids
$IV.$ Vitamins

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The nutrient medium for tissue culture should have:$I.$ Sucrose$II.$ Inorganic Salts$III.$ Amino acids$IV.$ Vitamins