The steps involved in the Southern blot test are as follows:
$I.$ $X-ray$ film
$II.$ Electrophoresis
$III.$ Digestion with restriction enzyme
$IV.$ Ethidium bromide
$V.$ Radioactive probe
Choose the option having the correct sequential order of these events.

The entry of $t-DNA$ into plants is associated with which of the following?

In $PCR$ technique,how many copies of the $DNA$ sample will be obtained after $6$ cycles are completed?

$I. ... A...$ is the ability of a cell to take up foreign $DNA$.
$II.$ The cell is treated with a specific concentration of a divalent cation such as $... B...$ to increase the pore size in the cell wall.
$III.$ In $...C...$ method,recombinant $DNA$ is directly injected into the nucleus of an animal cell.
The most appropriate option regarding $A, B$ and $C$ is:

The $Polyethylene \text{ } Glycol$ $(PEG)$ method is used for:

Which one of the following statements is wrong with respect to separation of $DNA$ fragments on gel electrophoresis?