The technique involving the insertion of a desired gene into the $DNA$ of a plasmid vector is known as:

Transformation is a process in which ..........

What is the significance of adding proteases at the time of isolation of genetic material $(DNA)$?

$mRNA$ silencing is known as .........

In this technique,large quantities of biotechnological products such as antibiotics,vaccines,and enzymes are produced by maintaining and growing only the desired microbes/eukaryotic cells in a sterile environment.

Assertion : The uptake of $DNA$ during transformation is an active,energy-requiring process.
Reason : Transformation occurs in only those bacteria,which possess the enzymatic machinery involved in the active uptake and recombination.