What are the requirements in tissue culture?

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(D) Tissue culture is a technique of growing plant cells,tissues,or organs in a sterile,artificial nutrient medium under controlled laboratory conditi

Vedclass · Accepted Answer

All of these

Vedclass · Answer

(D) Tissue culture is a technique of growing plant cells,tissues,or organs in a sterile,artificial nutrient medium under controlled laboratory conditions.To support the growth and development of the explant,the culture medium must contain essential nutrients.These requirements include: $1$. Inorganic salts (macro and micronutrients).$2$. Carbon sources (e.g.,sucrose).$3$. Vitamins and amino acids.$4$. Growth regulators (phytohormones) such as auxins and cytokinins.$5$. Solidifying agents like agar-agar (if a solid medium is required).Since all the options listed are essential components of the culture medium,the correct answer is $D$.

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