What is the first step in the Southern Blotti | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(A) The Southern Blotting technique is a fundamental method used in molecular biology for the detection of a specific $DNA$ sequence in $DNA$ samples.

Vedclass · Accepted Answer

Isolation of $DNA$ from a nucleated cell such as the one from the scene of crime

Vedclass · Answer

(A) The Southern Blotting technique is a fundamental method used in molecular biology for the detection of a specific $DNA$ sequence in $DNA$ samples. The steps involved are as follows:$1$. Isolation of $DNA$: The first step is the extraction and purification of $DNA$ from a biological sample (e.g.,blood,hair follicles,or tissue).$2$. Digestion: The isolated $DNA$ is then digested using restriction endonucleases to produce $DNA$ fragments of varying lengths.$3$. Gel Electrophoresis: These fragments are separated based on their size using agarose gel electrophoresis.$4$. Denaturation: The double-stranded $DNA$ fragments are denatured into single-stranded $DNA$ using an alkaline solution.$5$. Blotting: The single-stranded $DNA$ is transferred (blotted) onto a nitrocellulose or nylon membrane.$6$. Hybridization: The membrane is incubated with a labeled probe that binds to the complementary $DNA$ sequence.Therefore,the very first step is the isolation of $DNA$ from the source material.

What is the first step in the Southern Blotting technique?

Similar Questions

When $MgCl_2 \cdot 6H_2O$ is strongly heated,then it forms

The periodic time of a particle doing simple harmonic motion is $4 \ s$. The time taken by it to go from its mean position to half the maximum displacement (amplitude) is

The plant part which consists of two generations,one within the other,is:

Which of the following statements is not correct regarding calcination?

Vedclass Test Series

Exam Paper Generator

Online Exam Module