What is the first step in the Southern blot technique?

  • A
    Denaturation of $DNA$ on the gel for hybridization with specific probe
  • B
    Production of a group of genetically identical cells
  • C
    Digestion of $DNA$ by restriction enzyme
  • D
    Denaturation of $DNA$ from a nucleated cell such as the one from the scene of crime

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$I.$ Separation of the product from the reactor
$II.$ Purification of the product
$III.$ Formulation of the product with suitable preservatives
$IV.$ Quality control testing and clinical trials in case of drugs
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$(a)$ Origin of replication
$(b)$ Bioreactors
$(c)$ Downstream processing

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$PCR$ is a useful tool for the early diagnosis of an infectious disease. Elaborate.

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