What modification is done on the Ti plasmid o | vedclass

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(A) The tumor-inducing $(Ti)$ plasmid of $Agrobacterium$ $tumefaciens$ is modified by removing the $T-DNA$ region,which is responsible for tumor forma

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Removal of the $T-DNA$ region

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(A) The tumor-inducing $(Ti)$ plasmid of $Agrobacterium$ $tumefaciens$ is modified by removing the $T-DNA$ region,which is responsible for tumor formation in plants.This process is known as 'disarming' the plasmid.By removing the $T-DNA$ and replacing it with the gene of interest,the plasmid becomes a non-pathogenic cloning vector.It retains the ability to transfer the desired gene into plant cells using its natural $vir$ $(virulence)$ gene machinery.

What modification is done on the $Ti$ plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?

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