Which is the evidence to show that O_2 releas | vedclass

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(D) The evidence that $O_2$ released in photosynthesis comes from water is supported by multiple observations:$1$. $C.B. 	ext{ van Niel}$ demonstrate

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(D) The evidence that $O_2$ released in photosynthesis comes from water is supported by multiple observations:$1$. $C.B. 	ext{ van Niel}$ demonstrated that photosynthetic bacteria use $H_2S$ instead of $H_2O$ to produce carbohydrates, releasing sulfur instead of oxygen, which suggested that $O_2$ in plants comes from $H_2O$.$2$. The use of isotopic oxygen $(^{18}O)$ in $H_2O$ showed that the $O_2$ released during photosynthesis is labeled with the same isotope, confirming its origin from water.$3$. The Hill reaction showed that isolated chloroplasts can evolve $O_2$ in the presence of an artificial electron acceptor (like potassium ferrocyanide) when illuminated, even without $CO_2$ fixation, proving that water splitting (photolysis) is the source of $O_2$.

Which is the evidence to show that $O_2$ released in photosynthesis comes from water?

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