Which of the following is a main challenge fo | vedclass

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(D) In humans,insulin is synthesized as a pro-hormone containing an extra stretch called the $C$-peptide.This $C$-peptide is not present in the mature

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Assembling of insulin into mature insulin

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(D) In humans,insulin is synthesized as a pro-hormone containing an extra stretch called the $C$-peptide.This $C$-peptide is not present in the mature insulin and is removed during maturation.The main challenge for the production of insulin using $r-DNA$ technology was getting insulin assembled into a mature form.Eli Lilly,an American company,prepared two $DNA$ sequences corresponding to $A$ and $B$ chains of human insulin and introduced them in plasmids of $E. coli$ to produce insulin chains.These chains were then extracted and combined by creating disulfide bonds to form mature human insulin.

Which of the following is a main challenge for the production of insulin by the $r-DNA$ techniques?

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