Which of the following is correct for polymerase chain reaction $(\text{PCR})$?

  • A
    In $\text{PCR}$ multiple copies of $\text{DNA}$ are synthesized in the cell.
  • B
    The correct order of steps is denaturation,extension,and annealing,respectively.
  • C
    The $\text{DNA}$ probe used is complementary to the $5'$ end of the template strand.
  • D
    The $\text{DNA}$ polymerase used in $\text{PCR}$ is thermostable and remains active during high temperatures.

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