Which of the following is not used for isolation of $\text{DNA}$ from cells?

Which restriction enzymes have recognition sites within the antibiotic resistance genes $amp^R$ and $tet^R$ in the plasmid $pBR322$?

Which one of the following palindromic base sequences in $DNA$ can be easily cut at about the middle by some particular restriction enzyme?

In bacteria,genes for antibiotic resistance are usually located in:

$I.$ $Ori$ also controls the copy number of the linked $DNA$.
$II.$ If a foreign $DNA$ ligates at the $BamHI$ site of the tetracycline resistance gene in the vector $pBR322$,the recombinant plasmid loses the tetracycline resistance due to the insertion of foreign $DNA$.
Choose the correct option regarding the above statements.

$DNA$ from plant or animal tissues and bacterial cells can be obtained by treatment with appropriate enzymes. Match the following columns correctly.

Column-$I$	Column-$II$
$P$. Bacteria	$I$. Lysozyme
$Q$. Fungi	$II$. Cellulase
$R$. Plant cells	$III$. Chitinase