Which of the following would not be used in preparing recombinant $DNA$?

What is a palindromic sequence?

The ability to transform normal cells into cancerous cells is found in

The enzyme known as restriction endonuclease:

Given below are two statements: one is labelled as Assertion $(A)$ and the other is labelled as Reason $(R)$.
Assertion $(A)$: When a particular restriction enzyme cuts a strand of $DNA$,overhanging stretches or sticky ends are formed.
Reason $(R)$: Some restriction enzymes cut the strand of $DNA$ a little away from the centre of the palindromic site.
In the light of the above statements,choose the correct answer from the options given below:

$A$ chimaeric $DNA$ is formed by