E. coli cells were allowed to replicate in a | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(B) $DNA$ replication is semi-conservative in nature.When $E. coli$ cells are grown in a medium containing radioactive thymidine,the newly synthesized

Vedclass · Accepted Answer

One strand will be radioactive.

Vedclass · Answer

(B) $DNA$ replication is semi-conservative in nature.When $E. coli$ cells are grown in a medium containing radioactive thymidine,the newly synthesized $DNA$ strands incorporate the radioactive label.After $5$ minutes,the $DNA$ molecules will have one radioactive strand (template) and one newly synthesized radioactive strand (if replication occurred) or just the labeled template.When these cells are transferred to a normal medium,the new strands synthesized will use non-radioactive nucleotides.Since the original $DNA$ molecule had one radioactive strand and one non-radioactive strand (or both radioactive depending on the duration),the resulting daughter $DNA$ molecules will retain the radioactive strand as a template.Specifically,in this experiment,one strand of the $DNA$ duplex will remain radioactive because it serves as the template for the new,non-radioactive strand.Therefore,one strand of the $DNA$ will be radioactive.

$E. coli$ cells were allowed to replicate in a medium containing radioactive thymidine for $5$ minutes and then transferred to a normal medium for further replication. Which of the following observations will be correct?

Similar Questions

Eukaryotes differ from prokaryotes in the mechanism of $DNA$ replication due to

Depending upon the chemical nature of the template ($DNA$ or $RNA$) and the nature of nucleic acids synthesized from it ($DNA$ or $RNA$),list the types of nucleic acid polymerases.

At the end of replication,the contribution of nucleotides from mother $DNA$ is . . . . . . percent.

In which medium did Meselson and Stahl first culture $E. coli$?

During in vitro synthesis of $DNA$,a researcher used $2', 3'$-dideoxy cytidine triphosphate as raw nucleotide in place of $2'$-deoxy cytidine. What would be the consequence?

Vedclass Test Series

Exam Paper Generator

Online Exam Module