Which of the following is not used in the Sou | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(D) Southern hybridization is a technique used for the detection of specific $DNA$ sequences in $DNA$ samples.The steps involved in Southern hybridiza

Vedclass · Accepted Answer

$PCR$

Vedclass · Answer

(D) Southern hybridization is a technique used for the detection of specific $DNA$ sequences in $DNA$ samples.The steps involved in Southern hybridization are:$1$. Digestion of $DNA$ with restriction endonucleases.$2$. Separation of $DNA$ fragments by gel electrophoresis.$3$. Transfer of separated $DNA$ fragments onto a nitrocellulose or nylon membrane (Blotting).$4$. Hybridization with a labeled probe.$5$. Detection of the hybridized probe using autoradiography.$PCR$ (Polymerase Chain Reaction) is a separate technique used for the amplification of specific $DNA$ sequences and is not a step in the standard Southern hybridization procedure.

Which of the following is not used in the Southern hybridization technique for chromosomal $DNA$ analysis?

Similar Questions

In the case of polymerase chain reaction $(PCR)$,what are the temperatures required for the following steps?
$A.$ Denaturation
$B.$ Annealing
$C.$ Extension

$A$ mixture of fragmented $DNA$ was electrophoresed in an agarose gel. After staining the gel with ethidium bromide,no $DNA$ bands were observed. What could be the reason?

In a chemical process,the $DNA$ fragments produced by restriction endonucleases can be separated by:

In the presence of a chromogenic substrate,what color colonies do recombinants and non-recombinants produce,respectively?

The process of joining together different $DNA$ fragments is often referred to as

Vedclass Test Series

Exam Paper Generator

Online Exam Module