Describe the steps involved in $DNA$ fingerprinting.

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(N/A) $DNA$ fingerprinting involves identifying differences in specific regions in $DNA$ sequence called repetitive $DNA$. The steps are as follows:
$1$. Isolation of $DNA$.
$2$. Digestion of $DNA$ by restriction endonucleases.
$3$. Separation of $DNA$ fragments by electrophoresis.
$4$. Transferring (blotting) of separated $DNA$ fragments to synthetic membranes,such as nitrocellulose or nylon.
$5$. Hybridization using labelled $VNTR$ probe.
$6$. Detection of hybridized $DNA$ fragments by autoradiography.
$DNA$ fingerprinting was developed by Alec Jeffreys. It uses $VNTR$ (Variable Number of Tandem Repeats) as a probe because they show high degree of polymorphism.

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Similar Questions

Arrange the following steps of $DNA$ fingerprinting in a correct sequence.
$A$. Isolation of $DNA$ and its digestion by restriction endonucleases.
$B$. Hybridisation using a labelled $VNTR$ probe.
$C$. Transferring of separated $DNA$ fragments to synthetic membranes.
$D$. Detection of hybridised $DNA$ fragments by autoradiography.
$E$. Separation of $DNA$ fragments by electrophoresis.
Choose the correct answer from the options given below:

Which of the following is used in the $DNA$ fingerprinting technique?

What is $DNA$ fingerprinting?

The $DNA$ fingerprint pattern of a child is:

Select the $INCORRECT$ statement with reference to $DNA$ fingerprinting. It is . . . . . . .

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