At what time intervals did Meselson and Stahl | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(C) Meselson and Stahl performed an experiment to prove semi-conservative $DNA$ replication. They grew $E. coli$ in a medium containing $^{15}N$ (a he

Vedclass · Accepted Answer

$20$ minutes

Vedclass · Answer

(C) Meselson and Stahl performed an experiment to prove semi-conservative $DNA$ replication. They grew $E. coli$ in a medium containing $^{15}N$ (a heavy isotope of nitrogen) for many generations to label the $DNA$. Then, they transferred these bacteria into a medium containing $^{14}NH_{4}Cl$ (a normal isotope of nitrogen). Since $E. coli$ divides every $20$ minutes, they took samples at definite time intervals of $20$ minutes to analyze the density of the $DNA$ using cesium chloride $(CsCl)$ density gradient centrifugation.

At what time intervals did Meselson and Stahl take samples after transferring the bacteria to a $^{14}NH_{4}Cl$ medium?

Similar Questions

If $E. coli$ is allowed to grow for $40$ minutes in a medium containing $^{15}N$,then the number of $^{14}N / ^{14}N$ containing $DNA$ molecules would be:

$A$ $N^{15}-N^{15}$ $\text{DNA}$ molecule is continuously replicated in $N^{14}$ medium,then what is the percentage of heavy $\text{DNA}$ in the $4^{\text{th}}$ generation (in $\%$)?

Polymerisation of $DNA$ nucleotides during the synthesis of the lagging strand occurs in:

The process of $DNA$ replication is catalyzed by an enzyme called

During $DNA$ replication, the term leading template is applied to the one which replicates itself in $A$ and the replication is $B$.

Vedclass Test Series

Exam Paper Generator

Online Exam Module